PrimePCR™ Probe Assay: Tead3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Tead3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Tead3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053626
Assay Design:   exonic
Chromosome Location:   17:28332701-28332802question
Amplicon Length:   72
Splice Variants Targeted:   ENSMUST00000154873 ENSMUST00000114799 ENSMUST00000156862 ENSMUST00000080572 ENSMUST00000019363

Gene Information

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors which contain the TEA/ATTS DNA-binding domain. It is predominantly expressed in the placenta and thought to play a role in placental gene regulation and development. Alternative splicing and alternate use of an upstream AUG translation initiation codon and an in-frame downstream non-AUG (AUA) codon results in 2 isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   Tead3
Gene Name:   TEA domain family member 3
Aliases:   DTEF-1, ETFR-1, TEAD-3, TEF-5, Tcf13r2
RefSeq:   NC_000083.6 NT_039649.8 NT_187005.1
Ensembl:   ENSMUSG00000002249
Entrez:   21678
Chromosome Mapping:   17 A3.3|17 8.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.750000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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