PrimePCR™ Probe Assay: Blmh, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Blmh, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Blmh, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053840
Assay Design:   exonic
Chromosome Location:   11:76945881-76946337question
Amplicon Length:   79
Splice Variants Targeted:   ENSMUST00000140781 ENSMUST00000021197 ENSMUST00000155053 ENSMUST00000146197

Gene Information

The encoded protein is a cytoplasmic cysteine peptidase involved in inactivation of bleomycin a glycopeptide which is a component of combination chemotherapy regimens for cancer. This encoded enzyme is highly conserved and it contains the signature active site residues of cysteine protease papain superfamily enzymes. It is postulated that this enzyme has protective effects against bleomycin-induced pulmonary fibrosis and bleomycin tumor resistance. [provided by RefSeq Jan 2010]

Gene Symbol:   Blmh
Gene Name:   Bleomycin hydrolase
Aliases:   AI035728, Bh, Bmh
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000020840
Entrez:   104184
Chromosome Mapping:   11 B5|11

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