PrimePCR™ Probe Assay: Sgk1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Sgk1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Sgk1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053869
Assay Design:   exonic
Chromosome Location:   10:21995523-21995856question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000120509 ENSMUST00000142174 ENSMUST00000150089 ENSMUST00000092673 ENSMUST00000020145 ENSMUST00000124350 ENSMUST00000100036 ENSMUST00000164659 ENSMUST00000119527 ENSMUST00000117675 ENSMUST00000148835 ENSMUST00000088276 ENSMUST00000051171 ENSMUST00000031555

Gene Information

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium sodium and chloride channels suggesting an involvement in the regulation of processes such as cell survival neuronal excitability and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq Sep 2009]

Gene Symbol:   Sgk1
Gene Name:   Serum/glucocorticoid regulated kinase 1
Aliases:   Sgk
RefSeq:   NC_000076.6 NT_039492.8
Ensembl:   ENSMUSG00000019970
Entrez:   20393
Chromosome Mapping:   10 A3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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