PrimePCR™ Probe Assay: Dnmt3b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Dnmt3b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Dnmt3b, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054063
Assay Design:   exonic
Chromosome Location:   2:153665358-153665997question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000072997 ENSMUST00000109773 ENSMUST00000109774 ENSMUST00000081628 ENSMUST00000103151 ENSMUST00000088976 ENSMUST00000109772 ENSMUST00000103150 ENSMUST00000109771 ENSMUST00000056495

Gene Information

This is one of two related genes encoding de novo DNA methyltransferases which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq Nov 2012]

Gene Symbol:   Dnmt3b
Gene Name:   DNA methyltransferase 3B
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027478
Entrez:   13436
Chromosome Mapping:   2|2 A2-A3

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