PrimePCR™ Probe Assay: Nfasc, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Nfasc, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nfasc, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054296
Assay Design:   exonic
Chromosome Location:   1:132569511-132569621question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000094569 ENSMUST00000163770 ENSMUST00000043189

Gene Information

This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth neurite fasciculation and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein gliomedin ankyrin 3 (ankyrin-G) and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing and the full-length nature of some variants has not been determined. [provided by RefSeq May 2009]

Gene Symbol:   Nfasc
Gene Name:   Neurofascin
Aliases:   AA387016, D430023G06Rik, NF, mKIAA0756
RefSeq:   NC_000067.6 NT_078297.7
Ensembl:   ENSMUSG00000026442
Entrez:   269116
Chromosome Mapping:   1 E4|1 70.0 cM

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