PrimePCR™ Probe Assay: Plk4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Plk4, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Plk4, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054948
Assay Design:   exonic
Chromosome Location:   3:40801967-40805014question
Amplicon Length:   83
Splice Variants Targeted:   ENSMUST00000026858 ENSMUST00000170825 ENSMUST00000168287 ENSMUST00000167556 ENSMUST00000169566

Gene Information

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2 to centrosomes during G2/M and to the cleavage furrow during cytokinesis. It is required for progression through mitosis cell survival and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   Plk4
Gene Name:   Polo-like kinase 4 (Drosophila)
Aliases:   1700028H20, AI385771, Sak, Stk18
RefSeq:   NC_000069.6 NT_039229.7
Ensembl:   ENSMUSG00000025758
Entrez:   20873
Chromosome Mapping:   3 B|3

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