PrimePCR™ Probe Assay: Pou5f1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054951
Assay Design:   exonic
Chromosome Location:   17:35509983-35510075question
Amplicon Length:   63
Splice Variants Targeted:   ENSMUST00000025271 ENSMUST00000173934 ENSMUST00000172651 ENSMUST00000173805 ENSMUST00000173256 ENSMUST00000174782 ENSMUST00000174207

Gene Information

Description Not Available

Gene Symbol:   Pou5f1
Gene Name:   POU domain, class 5, transcription factor 1
Aliases:   NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
RefSeq:   NC_000083.6 NT_039649.8 NT_039662.3 NT_187027.1
Ensembl:   ENSMUSG00000024406
Entrez:   18999
Chromosome Mapping:   17 B1|17 19.23 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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