PrimePCR™ Probe Assay: Rprd1b, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rprd1b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rprd1b, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055242
Assay Design:   exonic
Chromosome Location:   2:158047944-158050091question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000090040 ENSMUST00000034267 ENSMUST00000103123 ENSMUST00000109518 ENSMUST00000029180 ENSMUST00000152452 ENSMUST00000128206 ENSMUST00000027961 ENSMUST00000046972 ENSMUST00000091920 ENSMUST00000120929 ENSMUST00000080233 ENSMUST00000111868 ENSMUST00000087657 ENSMUST00000038661 ENSMUST00000044664 ENSMUST00000169168 ENSMUST00000175866 ENSMUST00000177488 ENSMUST00000176383 ENSMUST00000026284 ENSMUST00000025862 ENSMUST00000176030 ENSMUST00000176769 ENSMUST00000109428 ENSMUST00000099110 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rprd1b
Gene Name:   Regulation of nuclear pre-mRNA domain containing 1B
Aliases:   2610304G08Rik, 2810446G03Rik, Crept
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027651
Entrez:   70470
Chromosome Mapping:   2|2 H2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.530000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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