PrimePCR™ Probe Assay: Bag1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Bag1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Bag1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055319
Assay Design:   exonic
Chromosome Location:   4:40936551-40936665question
Amplicon Length:   85
Splice Variants Targeted:   ENSMUST00000108089 ENSMUST00000030125

Gene Information

The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to Bcl2 protein and is referred to as Bcl2-associated athanogene. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative downstream AUG translation initiation site. [provided by RefSeq Jul 2008]

Gene Symbol:   Bag1
Gene Name:   BCL2-associated athanogene 1
Aliases:   BAG-1, Rap46
RefSeq:   NC_000070.6 NT_187032.1
Ensembl:   ENSMUSG00000028416
Entrez:   12017
Chromosome Mapping:   4|4 B1

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