PrimePCR™ Probe Assay: Casp4, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0056026
Assay Design:   exonic
Chromosome Location:   9:5322820-5323631question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000162846 ENSMUST00000027012

Gene Information

This gene encodes a caspase that is involved in apoptosis cell migration and the inflammatory response. Although its official nomenclature is Casp4 caspase 4 apoptosis-related cysteine peptidase this gene and its encoded protein have historically been called caspase 11. [provided by RefSeq Feb 2009]

Gene Symbol:   Casp4
Gene Name:   Caspase 4, apoptosis-related cysteine peptidase
Aliases:   Casp11, Caspl, ich-3
RefSeq:   NC_000075.6 NT_039471.8
Ensembl:   ENSMUSG00000033538
Entrez:   12363
Chromosome Mapping:   9 A1|9 1.1 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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