PrimePCR™ Probe Assay: Mars, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Mars, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Mars, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057588
Assay Design:   exonic
Chromosome Location:   10:127296340-127296439question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000037290 ENSMUST00000134778 ENSMUST00000171564

Gene Information

The encoded protein belongs to the class I family of tRNA synthetases a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement conserved in human and mouse may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants.[provided by RefSeq Jan 2010]

Gene Symbol:   Mars
Gene Name:   Methionine-tRNA synthetase
Aliases:   Metrs, Mtrns
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000040354
Entrez:   216443
Chromosome Mapping:   10 D3|10

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