PrimePCR™ Probe Assay: Stmn1, Mouse

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: Stmn1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: Stmn1, Mouse
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qMmuCEP0062662
Assay Design:		exonic
Chromosome Location:		4:134470824-134472879question
Amplicon Length:		62
Splice Variants Targeted:		ENSMUST00000120367 ENSMUST00000060513 ENSMUST00000086248 ENSMUST00000155534 ENSMUST00000163321 ENSMUST00000041924 ENSMUST00000003468 ENSMUST00000073277 ENSMUST00000101205 ENSMUST00000031189 ENSMUST00000119014 ENSMUST00000118910 ENSMUST00000030043 ENSMUST00000025847 ENSMUST00000060714 ENSMUST00000025728 ENSMUST00000165096 ENSMUST00000035336 ENSMUST00000092684 ENSMUST00000006976 ENSMUST00000037141 ENSMUST00000025778 ENSMUST00000030636 ENSMUST00000127279 ENSMUST00000105867 ENSMUST00000105868

Gene Information

Description Not Available

Gene Symbol:		Stmn1
Gene Name:		Stathmin 1
Aliases:		19k, Lag, Lap18, Op18, P18, P19, Pig, Pp17, Pp18, Pp19, Pr22, Smn
RefSeq:		NC_000070.6 NT_187033.1
Ensembl:		ENSMUSG00000028832
Entrez:		16765
Chromosome Mapping:		4 D3\|4 65.7 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	36.230000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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