PrimePCR™ Probe Assay: Cldn15, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cldn15, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cldn15, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0028540
Assay Design:   Intron-spanning
Chromosome Location:   5:136968209-136972410question
Amplicon Length:   176
Splice Variants Targeted:   ENSMUST00000111093 ENSMUST00000001790

Gene Information

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets and also play critical roles in maintaining cell polarity and signal transductions. This protein increases permeability for sodium ions in anion-selective epithelial cell sheets. The gene deficiency leads to megaintestine and decreases in intestinal epithelial paracellular ion permeability. This gene is a direct target for hepatocyte-nuclear-factor-4alpha a mediator of ion epithelial transport and is down-modulated in inflammatory bowel disease. [provided by RefSeq Aug 2010]

Gene Symbol:   Cldn15
Gene Name:   Claudin 15
Aliases:   2210009B08Rik, BB107105
RefSeq:   NC_000071.6 NT_039314.8
Ensembl:   ENSMUSG00000001739
Entrez:   60363
Chromosome Mapping:   5 G2|5

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