PrimePCR™ Probe Assay: Aanat, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Aanat, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Aanat, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0029672
Assay Design:   Intron-spanning
Chromosome Location:   11:116593726-116595701question
Amplicon Length:   145
Splice Variants Targeted:   ENSMUST00000153476

Gene Information

The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:   Aanat
Gene Name:   Arylalkylamine N-acetyltransferase
Aliases:   AA-NAT, Nat-2, Nat4, Snat
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000020804
Entrez:   11298
Chromosome Mapping:   11 E2|11 70.0 cM

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