PrimePCR™ Probe Assay: Aspa, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Aspa, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Aspa, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030028
Assay Design:   Intron-spanning
Chromosome Location:   11:73319857-73322193question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000021119 ENSMUST00000134079

Gene Information

This gene encodes an enzyme that deacteylates N-acetyl-L-aspartic acid (NAA) in the brain to yield acetate and L-aspartate. In humans alterations in neuronal NAA concentration are associated with many neurodegenerative diseases (decrease associated with epilepsy multiple sclerosis myotrophic lateral sclerosis and Alzheimer's disease; increase associated with Canavan disease). In mouse mutations in this gene which cause accumulation of NAA result in demyelination and spongy degeneration in the CNS and serve as a pathophysiological model for Canavan disease. [provided by RefSeq Dec 2012]

Gene Symbol:   Aspa
Gene Name:   Aspartoacylase
Aliases:   Acy-2, Acy2, nur7
RefSeq:   NC_000077.6 NT_096135.6 NT_166317.2
Ensembl:   ENSMUSG00000020774
Entrez:   11484
Chromosome Mapping:   11 B4|11

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