PrimePCR™ Probe Assay: Crbn, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Crbn, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Crbn, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030420
Assay Design:   Intron-spanning
Chromosome Location:   6:106790873-106794211question
Amplicon Length:   113
Splice Variants Targeted:   ENSMUST00000049675 ENSMUST00000113239 ENSMUST00000013882

Gene Information

This gene encodes a protein with a Lon protease domain a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic mental retardation. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq Jan 2013]

Gene Symbol:   Crbn
Gene Name:   Cereblon
Aliases:   2610203G15Rik, 2900045O07Rik, AF229032, AW108261, piL
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000005362
Entrez:   58799
Chromosome Mapping:   6 E2|6

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