PrimePCR™ Probe Assay: Irf1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030543
Assay Design:   Intron-spanning
Chromosome Location:   11:53771317-53772929question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000108920 ENSMUST00000140866 ENSMUST00000108922 ENSMUST00000138913 ENSMUST00000123376 ENSMUST00000019043 ENSMUST00000133291

Gene Information

Description Not Available

Gene Symbol:   Irf1
Gene Name:   Interferon regulatory factor 1
Aliases:   AU020929, Irf-1
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000018899
Entrez:   16362
Chromosome Mapping:   11 B1.3|11 29.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.150000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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