PrimePCR™ Probe Assay: Abcg5, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Abcg5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Abcg5, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030694
Assay Design:   Intron-spanning
Chromosome Location:   17:84669007-84669962question
Amplicon Length:   90
Splice Variants Targeted:   ENSMUST00000066175 ENSMUST00000163375

Gene Information

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1 MDR/TAP MRP ALD OABP GCN20 White). This protein is a member of the White subfamily. In humans this protein functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols; however the function of the mouse gene has not been determined. Mutations in the human gene have been associated with sterol accumulation atheroschlerosis and sitosterolemia. [provided by RefSeq Jul 2008]

Gene Symbol:   Abcg5
Gene Name:   ATP-binding cassette, sub-family G (WHITE), member 5
Aliases:   AW112016, cmp, sterolin-1, trac
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000040505
Entrez:   27409
Chromosome Mapping:   17 E4|17 54.5 cM

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