PrimePCR™ Probe Assay: Wt1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Wt1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Wt1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0030838
Assay Design:   Intron-spanning
Chromosome Location:   2:105169971-105172306question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000143043 ENSMUST00000146842 ENSMUST00000139585 ENSMUST00000111099 ENSMUST00000111098

Gene Information

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system and the orthologous human gene is mutated in a small subset of patients with Wilm's tumors. Alternative splicing has been noted for this gene however the full-length nature of these variants is not known. The mRNA for this gene has been shown to initiate translation from non-AUG (CUG) and AUG translation start sites resulting in different isoforms. This gene has also been reported to be imprinted with preferential expression from the maternal allele. [provided by RefSeq Dec 2010]

Gene Symbol:   Wt1
Gene Name:   Wilms tumor 1 homolog
Aliases:   D630046I19Rik, Wt-1
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000016458
Entrez:   22431
Chromosome Mapping:   2 E|2 58.0 cM

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