PrimePCR™ Probe Assay: Snap91, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $220.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0031257
Assay Design:   Intron-spanning
Chromosome Location:   9:86835163-86836686question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000074501 ENSMUST00000074468 ENSMUST00000036347 ENSMUST00000098495 ENSMUST00000167014

Gene Information

Description Not Available

Gene Symbol:   Snap91
Gene Name:   Synaptosomal-associated protein 91
Aliases:   91kDa, AP180, F1-20, mKIAA0656
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000033419
Entrez:   20616
Chromosome Mapping:   9 E3.2|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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