PrimePCR™ Probe Assay: Dcx, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Dcx, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Dcx, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0031581
Assay Design:   Intron-spanning
Chromosome Location:   X:143923345-143930979question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000033642 ENSMUST00000112851 ENSMUST00000087313 ENSMUST00000112856

Gene Information

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains which bind microtubules. In the developing cortex cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition the encoded protein interacts with LIS1 the regulatory gamma subunit of platelet activating factor acetylhydrolase. Studies in knockout mice lacking this gene and the LIS1 gene suggest that the molecular interaction of these two genes is important in both in neuronal migration and neurogenesis and there is a cortical role of this gene in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. Multiple transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq Sep 2010]

Gene Symbol:   Dcx
Gene Name:   Doublecortin
Aliases:   Dbct
RefSeq:   NC_000086.7 NT_039718.8
Ensembl:   ENSMUSG00000031285
Entrez:   13193
Chromosome Mapping:   X F2|X

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