PrimePCR™ Probe Assay: Spc25, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: Spc25, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Spc25, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033444
Assay Design:   Intron-spanning
Chromosome Location:   2:69200038-69202628question
Amplicon Length:   79
Splice Variants Targeted:   ENSMUST00000005365 ENSMUST00000127243 ENSMUST00000112320 ENSMUST00000149045 ENSMUST00000149643 ENSMUST00000167875

Gene Information

Description Not Available

Gene Symbol:   Spc25
Gene Name:   SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Aliases:   2600017H08Rik, 2610205L13Rik, Spbc25
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000005233
Entrez:   66442
Chromosome Mapping:   2|2 C3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.970000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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