PrimePCR™ Probe Assay: Acox1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Acox1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Acox1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033464
Assay Design:   Intron-spanning
Chromosome Location:   11:116175322-116178153question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000066587 ENSMUST00000072948 ENSMUST00000148601

Gene Information

This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis spontaneous peroxisome proliferation and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2012]

Gene Symbol:   Acox1
Gene Name:   Acyl-Coenzyme A oxidase 1, palmitoyl
Aliases:   AI042784, AOX, D130055E20Rik
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000020777
Entrez:   11430
Chromosome Mapping:   11 E2|11

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