PrimePCR™ Probe Assay: Nos3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Nos3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nos3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0033833
Assay Design:   Intron-spanning
Chromosome Location:   5:24372634-24377637question
Amplicon Length:   188
Splice Variants Targeted:   ENSMUST00000030834 ENSMUST00000115090

Gene Information

Description Not Available

Gene Symbol:   Nos3
Gene Name:   Nitric oxide synthase 3, endothelial cell
Aliases:   2310065A03Rik, Nos-3, eNOS, ecNOS
RefSeq:   NC_000071.6 NT_165760.3
Ensembl:   ENSMUSG00000028978
Entrez:   18127
Chromosome Mapping:   5 A3|5 9.0 cM

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