PrimePCR™ Probe Assay: Trim24, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Trim24, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Trim24, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034210
Assay Design:   Intron-spanning
Chromosome Location:   6:37903566-37908271question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000031859 ENSMUST00000120428 ENSMUST00000120238

Gene Information

The protein encoded by this gene is part of the tripartite-motif containing family (TRIM) which are typified by the RING B-box type 1 B-box type 2 and coiled-coil region domains. This protein which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin has important roles in differentiation development and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene which results in the fusion protein T18 has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq Jan 2013]

Gene Symbol:   Trim24
Gene Name:   Tripartite motif-containing 24
Aliases:   A130082H20Rik, AI447469, D430004I05Rik, TIF1, TIF1alpha, Tif1a
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000029833
Entrez:   21848
Chromosome Mapping:   6 B1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 34.850000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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