PrimePCR™ Probe Assay: Dclk1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Dclk1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Dclk1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034291
Assay Design:   Intron-spanning
Chromosome Location:   3:55256159-55380867question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000054237 ENSMUST00000167204

Gene Information

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains which bind microtubules and regulate microtubule polymerization a C-terminal serine/threonine protein kinase domain which shows substantial homology to Ca2+/calmodulin-dependent protein kinase and a serine/proline-rich domain in between the doublecortin and the protein kinase domains which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes including neuronal migration retrograde transport neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found but the biological validity of some variants has not been determined. These variants encode different isoforms which are differentially expressed and have different kinase activities. [provided by RefSeq Sep 2010]

Gene Symbol:   Dclk1
Gene Name:   Doublecortin-like kinase 1
Aliases:   1700113D08Rik, 2810480F11Rik, AI836758, Click-I, Cpg16, Dcamkl1, Dcl, Dclk, mKIAA0369
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000027797
Entrez:   13175
Chromosome Mapping:   3|3 D

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.370000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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