PrimePCR™ Probe Assay: Basp1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: Basp1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Basp1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0034801
Assay Design:   Intron-spanning
Chromosome Location:   15:25364873-25413725question
Amplicon Length:   190
Splice Variants Targeted:   ENSMUST00000058845 ENSMUST00000047075 ENSMUST00000047157 ENSMUST00000021802 ENSMUST00000119341 ENSMUST00000115094 ENSMUST00000115095 ENSMUST00000055483 ENSMUST00000026978

Gene Information

Description Not Available

Gene Symbol:   Basp1
Gene Name:   Brain abundant, membrane attached signal protein 1
Aliases:   2610024P12Rik, CAP-23, CAP23, Ckap3, NAP-22, NAP22
RefSeq:   NC_000081.6 NT_039618.8
Ensembl:   ENSMUSG00000045763
Entrez:   70350
Chromosome Mapping:   15 B1|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.240000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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