PrimePCR™ Probe Assay: Ddx17, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Ddx17, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Ddx17, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0037235
Assay Design:   Intron-spanning
Chromosome Location:   15:79539361-79540469question
Amplicon Length:   127
Splice Variants Targeted:   ENSMUST00000054014

Gene Information

This gene encodes the mouse homolog of human DEAD box polypeptide 17. DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). RNA helicases of the DEAD-box family are involved in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and splicesosome assembly. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   Ddx17
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
Aliases:   2610007K22Rik, A430025E01Rik, AI047725, C80929, Gm926, p72
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000055065
Entrez:   67040
Chromosome Mapping:   15 E1|15

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