PrimePCR™ Probe Assay: Cd300lh, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cd300lh, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: Cd300lh, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0062625
Assay Design:   Intron-spanning
Chromosome Location:   11:115046047-115048286question
Amplicon Length:   62
Splice Variants Targeted:   ENSMUST00000154689 ENSMUST00000102697 ENSMUST00000091246 ENSMUST00000057104 ENSMUST00000167263 ENSMUST00000080654 ENSMUST00000038701 ENSMUST00000067549 ENSMUST00000166169 ENSMUST00000135523 ENSMUST00000058326 ENSMUST00000019143 ENSMUST00000049248 ENSMUST00000070758 ENSMUST00000111334 ENSMUST00000045847 ENSMUST00000112498 ENSMUST00000120095 ENSMUST00000024829 ENSMUST00000029024 ENSMUST00000113337 ENSMUST00000113335 ENSMUST00000107511 ENSMUST00000107509 ENSMUST00000017339 ENSMUST00000107513 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Cd300lh
Gene Name:   CD300 antigen like family member H
Aliases:   AY457049, Clm3, Lmir7, clm-3
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000069607
Entrez:   382551
Chromosome Mapping:   11 E2|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.440000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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