PrimePCR™ Probe Assay: Thpo, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $225.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0022961
Assay Design:   exonic
Chromosome Location:   11:85931130-85931253question
Amplicon Length:   94
Splice Variants Targeted:   ENSRNOT00000073743 ENSRNOT00000072402

Gene Information

lineage-specific cytokine; important for proliferation and development of megakaryocytes and platelets; may be the major regulator of circulating platelets [RGD, Feb 2006]

Gene Symbol:   Thpo
Gene Name:   Thrombopoietin
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050172
Entrez:   81811
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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