PrimePCR™ Probe Assay: Aldh3a1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024220
Assay Design:   exonic
Chromosome Location:   10:47273957-47274049question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000003182

Gene Information

aldehyde dehydrogenase that is expressed during hepatocarcinogenesis [RGD, Feb 2006]

Gene Symbol:   Aldh3a1
Gene Name:   Aldehyde dehydrogenase, dimeric NADP-preferring
Aliases:   Not Available
RefSeq:   NM_031972
Ensembl:   ENSRNOG00000002331
Entrez:   25375
Chromosome Mapping:   10q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.210000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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