PrimePCR™ Probe Assay: Cxcr2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024344
Assay Design:   exonic
Chromosome Location:   9:81199196-81199341question
Amplicon Length:   116
Splice Variants Targeted:   ENSRNOT00000019109

Gene Information

chemokine receptor that binds Il8, GRO/MGSA and neutrophil activating peptide-2; involved in the inflammatory response [RGD, Feb 2006]

Gene Symbol:   Cxcr2
Gene Name:   C-X-C chemokine receptor type 2
Aliases:   Not Available
RefSeq:   NM_017183
Ensembl:   ENSRNOG00000014269
Entrez:   29385
Chromosome Mapping:   9q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.720000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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