PrimePCR™ Probe Assay: Cd247, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Cd247, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cd247, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0025747
Assay Design:   exonic
Chromosome Location:   13:88950313-88950539question
Amplicon Length:   197
Splice Variants Targeted:   ENSRNOT00000004403 ENSRNOT00000070958 ENSRNOT00000045130 ENSRNOT00000005654

Gene Information

component of T cell receptor (TCR) complex that plays a role in TCR assembly and signaling; does not promote surface expression of Fc gamma RIII, unlike human homolog [RGD, Feb 2006]

Gene Symbol:   Cd247
Gene Name:   T-cell surface glycoprotein CD3 zeta chain isoform 1 precursor
Aliases:   Not Available
RefSeq:   NM_170789 NM_001205304
Ensembl:   ENSRNOG00000003298
Entrez:   25300
Chromosome Mapping:   13q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.770000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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