PrimePCR™ Probe Assay: Enc1, Rat

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: Enc1, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: Enc1, Rat
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qRnoCEP0026067
Assay Design:		exonic
Chromosome Location:		2:47171137-47171283question
Amplicon Length:		117
Splice Variants Targeted:		ENSRNOT00000022144

Gene Information

mouse homolog binds actin and may play a role in nervous system induction and development [RGD, Feb 2006]

Gene Symbol:		Enc1
Gene Name:		Ectoderm-neural cortex protein 1
Aliases:		Not Available
RefSeq:		NM_001003401
Ensembl:		ENSRNOG00000016541
Entrez:		294674
Chromosome Mapping:		2q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	35.820000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761	PrimePCR™ Assays Quick Guide, Ver B	Click to download
10042030	PrimePCR™ PreAmp Assay Quick Guide, Rev A	Click to download
10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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