PrimePCR™ Probe Assay: Pygm, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0026436
Assay Design:   exonic
Chromosome Location:   1:228749983-228750082question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000028636

Gene Information

catalyzes the hydrolysis of intracellular glycogen [RGD, Feb 2006]

Gene Symbol:   Pygm
Gene Name:   Glycogen phosphorylase, muscle form
Aliases:   Not Available
RefSeq:   NM_012638
Ensembl:   ENSRNOG00000021090
Entrez:   24701
Chromosome Mapping:   1q43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.690000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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