PrimePCR™ Probe Assay: Gstm5, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0026934
Assay Design:   exonic
Chromosome Location:   2:230156658-230157002question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000073649 ENSRNOT00000072342

Gene Information

involved in conjugating GSH with a variety of electrophilic substrates [RGD, Feb 2006]

Gene Symbol:   Gstm5
Gene Name:   Glutathione S-transferase Mu 5
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049743
Entrez:   64352
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.840000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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