PrimePCR™ Probe Assay: Cxcl12, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0027211
Assay Design:   exonic
Chromosome Location:   4:215198681-215200889question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000032454 ENSRNOT00000066670 ENSRNOT00000071175 ENSRNOT00000064824

Gene Information

a chemoattractant molecule; involved in cell migration [RGD, Feb 2006]

Gene Symbol:   Cxcl12
Gene Name:   Chemokine (C-X-C motif) ligand 12 isoform alpha precursor
Aliases:   Not Available
RefSeq:   NM_001033883 NM_001033882 NM_022177
Ensembl:   ENSRNOG00000013589
Entrez:   24772
Chromosome Mapping:   4q42.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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