PrimePCR™ Probe Assay: Epor, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $225.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0027658
Assay Design:   exonic
Chromosome Location:   8:23024985-23025124question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000017369

Gene Information

binds erythropoietin and induces an increase in cytosolic calcium ion concentration [RGD, Feb 2006]

Gene Symbol:   Epor
Gene Name:   Erythropoietin receptor precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000012619
Entrez:   24336
Chromosome Mapping:   8q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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