PrimePCR™ Probe Assay: Chp, Rat

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: Chp, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: Chp, Rat
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qRnoCEP0028771
Assay Design:		exonic
Chromosome Location:		3:117937039-117937134question
Amplicon Length:		66
Splice Variants Targeted:		ENSRNOT00000056405

Gene Information

may act as a cellular Ca2+ signal transducer; required for exocytic membrane trafficking [RGD, Feb 2006]

Gene Symbol:		Chp
Gene Name:		Calcineurin B homologous protein 1
Aliases:		Not Available
RefSeq:		NM_024139
Ensembl:		ENSRNOG00000004742
Entrez:		64152
Chromosome Mapping:		3q35

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.940000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761	PrimePCR™ Assays Quick Guide, Ver B	Click to download
10042030	PrimePCR™ PreAmp Assay Quick Guide, Rev A	Click to download
10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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