PrimePCR™ Probe Assay: Ets1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0030391
Assay Design:   exonic
Chromosome Location:   8:33919794-33919925question
Amplicon Length:   102
Splice Variants Targeted:   ENSRNOT00000064687 ENSRNOT00000011925 ENSRNOT00000065428 ENSRNOT00000024428

Gene Information

activates transcription of cyclin-dependent kinase inhibitor p21WAF1/Cip1; plays a role in inhibition of apoptosis [RGD, Feb 2006]

Gene Symbol:   Ets1
Gene Name:   Protein C-ets-1
Aliases:   Not Available
RefSeq:   NM_012555
Ensembl:   ENSRNOG00000008941
Entrez:   24356
Chromosome Mapping:   8q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.390000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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