PrimePCR™ Probe Assay: Rps2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: Rps2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rps2, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031173
Assay Design:   exonic
Chromosome Location:   10:13904945-13905084question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000043480 ENSRNOT00000023108 ENSRNOT00000020091 ENSRNOT00000035389 ENSRNOT00000002656 ENSRNOT00000004583 ENSRNOT00000019508 ENSRNOT00000035386 ENSRNOT00000060074 ENSRNOT00000036337 ENSRNOT00000058491 ENSRNOT00000007876 ENSRNOT00000003897 ENSRNOT00000028014 ENSRNOT00000042870 ENSRNOT00000044776 ENSRNOT00000010437 ENSRNOT00000047772

Gene Information

40S ribosomal subunit protein [RGD, Feb 2006]

Gene Symbol:   Rps2
Gene Name:   40S ribosomal protein S2
Aliases:   Not Available
RefSeq:   NM_031838
Ensembl:   ENSRNOG00000014179
Entrez:   83789
Chromosome Mapping:   10q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 37.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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