PrimePCR™ Probe Assay: Clcn2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Clcn2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Clcn2, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0036942
Assay Design:   exonic
Chromosome Location:   11:85944299-85944593question
Amplicon Length:   113
Splice Variants Targeted:   ENSRNOT00000071707 ENSRNOT00000073435 ENSRNOT00000073841 ENSRNOT00000027609 ENSRNOT00000016768 ENSRNOT00000032223 ENSRNOT00000039228 ENSRNOT00000017965 ENSRNOT00000050927 ENSRNOT00000002376 ENSRNOT00000071956

Gene Information

has voltage-gated chloride channel activity; activity is also regulated by volume and pH; may play a role in chloride transport in many tissues [RGD, Feb 2006]

Gene Symbol:   Clcn2
Gene Name:   Chloride channel protein 2
Aliases:   Not Available
RefSeq:   NM_017137
Ensembl:   ENSRNOG00000050854
Entrez:   29232
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.210000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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