PrimePCR™ Probe Assay: Olr1481, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: Olr1481, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olr1481, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0039688
Assay Design:   exonic
Chromosome Location:   10:59744619-59744747question
Amplicon Length:   99
Splice Variants Targeted:   ENSRNOT00000010172 ENSRNOT00000026857 ENSRNOT00000071005 ENSRNOT00000074072 ENSRNOT00000072476 ENSRNOT00000060989 ENSRNOT00000072967 ENSRNOT00000072790 ENSRNOT00000045221 ENSRNOT00000074648 ENSRNOT00000071283 ENSRNOT00000072328 ENSRNOT00000075388 ENSRNOT00000071349 ENSRNOT00000071144 ENSRNOT00000072702

Gene Information

Description Not Available

Gene Symbol:   Olr1481
Gene Name:   Olfactory receptor Olr1481
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049457
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.140000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
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