PrimePCR™ Probe Assay: Nr3c1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $225.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0040497
Assay Design:   exonic
Chromosome Location:   18:32347604-32347747question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000019409

Gene Information

glucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]

Gene Symbol:   Nr3c1
Gene Name:   Glucocorticoid receptor
Aliases:   Not Available
RefSeq:   NM_012576
Ensembl:   ENSRNOG00000048800
Entrez:   24413
Chromosome Mapping:   18p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.250000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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