PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000050248, Rat

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000050248, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000050248, Rat
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qRnoCEP0042382
Assay Design:		exonic
Chromosome Location:		18:86782183-86782308question
Amplicon Length:		96
Splice Variants Targeted:		ENSRNOT00000071873 ENSRNOT00000070996 ENSRNOT00000072012 ENSRNOT00000072688 ENSRNOT00000074151 ENSRNOT00000073826 ENSRNOT00000071695 ENSRNOT00000074787 ENSRNOT00000074316 ENSRNOT00000048832 ENSRNOT00000049456 ENSRNOT00000073881 ENSRNOT00000071140 ENSRNOT00000071352 ENSRNOT00000075615 ENSRNOT00000075533 ENSRNOT00000072079 ENSRNOT00000075488 ENSRNOT00000073190 ENSRNOT00000072157 ENSRNOT00000074064 ENSRNOT00000073600 ENSRNOT00000073311 ENSRNOT00000073232 ENSRNOT00000074940 ENSRNOT00000072415 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000050248
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000050248
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	36.100000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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