PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000029224, Rat

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000029224, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000029224, Rat
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qRnoCEP0042647
Assay Design:		exonic
Chromosome Location:		15:20814506-20814638question
Amplicon Length:		103
Splice Variants Targeted:		ENSRNOT00000009426 ENSRNOT00000046984 ENSRNOT00000017635 ENSRNOT00000046053 ENSRNOT00000066489 ENSRNOT00000006211 ENSRNOT00000049646 ENSRNOT00000050478 ENSRNOT00000048281 ENSRNOT00000016042 ENSRNOT00000065546 ENSRNOT00000043147 ENSRNOT00000058640 ENSRNOT00000046997 ENSRNOT00000051642 ENSRNOT00000046785 ENSRNOT00000040837 ENSRNOT00000007793 ENSRNOT00000022114 ENSRNOT00000031497 ENSRNOT00000072831 ENSRNOT00000041628 ENSRNOT00000045504 ENSRNOT00000040676 ENSRNOT00000036049 ENSRNOT00000044787 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000029224
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000029224
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998600
y-intercept	36.300000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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