PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000049553, Rat

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000049553, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000049553, Rat
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qRnoCEP0044222
Assay Design:		exonic
Chromosome Location:		15:33446613-33446733question
Amplicon Length:		91
Splice Variants Targeted:		ENSRNOT00000072745 ENSRNOT00000074483 ENSRNOT00000075008 ENSRNOT00000073056 ENSRNOT00000071501 ENSRNOT00000071822 ENSRNOT00000074419 ENSRNOT00000067263 ENSRNOT00000072977 ENSRNOT00000072418 ENSRNOT00000073533 ENSRNOT00000072686 ENSRNOT00000075625 ENSRNOT00000070880 ENSRNOT00000073618 ENSRNOT00000060304 ENSRNOT00000042721 ENSRNOT00000071012 ENSRNOT00000072946 ENSRNOT00000071887 ENSRNOT00000060380

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000049553
Gene Name:		Protein LOC100360275; Uncharacterized protein
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000049553
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.690000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761	PrimePCR™ Assays Quick Guide, Ver B	Click to download
10042030	PrimePCR™ PreAmp Assay Quick Guide, Rev A	Click to download
10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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