PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000046538, Rat

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000046538, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000046538, Rat
Reaction: 4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qRnoCEP0044438
Assay Design:		exonic
Chromosome Location:		20:13745755-13745980question
Amplicon Length:		196
Splice Variants Targeted:		ENSRNOT00000047741 ENSRNOT00000002881 ENSRNOT00000042545 ENSRNOT00000041003 ENSRNOT00000066482 ENSRNOT00000001633 ENSRNOT00000008901 ENSRNOT00000025878 ENSRNOT00000022086 ENSRNOT00000034970 ENSRNOT00000041876 ENSRNOT00000018935 ENSRNOT00000016846 ENSRNOT00000003452 ENSRNOT00000021168 ENSRNOT00000051338 ENSRNOT00000035906 ENSRNOT00000071411 ENSRNOT00000020767 ENSRNOT00000014603 ENSRNOT00000042528 ENSRNOT00000064285 ENSRNOT00000043561 ENSRNOT00000054966 ENSRNOT00000002038 ENSRNOT00000025338 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000046538
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000046538
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	36.220000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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