PrimePCR™ Probe Assay: Spag5, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Spag5, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Spag5, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0047008
Assay Design:   Exonic
Chromosome Location:   10:66068264-66068407question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000015651

Gene Information

C-terminal has two leucine zippers which are involved in sperm tail protein interactions [RGD, Feb 2006]

Gene Symbol:   Spag5
Gene Name:   Sperm-associated antigen 5
Aliases:   Not Available
RefSeq:   NM_001044224
Ensembl:   ENSRNOG00000011777
Entrez:   252918
Chromosome Mapping:   10q25

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