PrimePCR™ Probe Assay: Vpreb1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Vpreb1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Vpreb1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0047210
Assay Design:   Exonic
Chromosome Location:   11:91363911-91364010question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000024978 ENSRNOT00000027315 ENSRNOT00000066566 ENSRNOT00000030569 ENSRNOT00000012056 ENSRNOT00000051934 ENSRNOT00000014269 ENSRNOT00000030604 ENSRNOT00000037660 ENSRNOT00000011208 ENSRNOT00000044477 ENSRNOT00000059127 ENSRNOT00000041811 ENSRNOT00000032861 ENSRNOT00000024443 ENSRNOT00000006786 ENSRNOT00000002882 ENSRNOT00000036206 ENSRNOT00000003061 ENSRNOT00000020711 ENSRNOT00000042865 ENSRNOT00000064798 ENSRNOT00000010609

Gene Information

mouse homolog forms a surrogate light chain in the pre-B cell receptor and plays a role in B-cell proliferation and expansion [RGD, Feb 2006]

Gene Symbol:   Vpreb1
Gene Name:   Pre-B lymphocyte 1 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000043140
Entrez:   303782
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.820000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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